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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

This protective effect of TP receptor knockout is achieved by activating ROCK1-mediated Drp1 phosphorylation and subsequent translocation into mitochondria, thereby inducing mitochondrial division

Fischer A, Sananbenesi F, Mungenast A, Tsai L-H (2010b) Targeting the correct HDAC(s) to treat cognitive disorders

and FCT (strategic project UID/NEU/04539/2013

This stimulates the production of collagen and elastin, two important proteins that help to firm and tighten the skin